SEPARATION OF HUMOR COMPONENT BY LIQUID CHROMATOGRAPHY

摘要

PURPOSE:To separate humor components simply, quickly, and precisely, by injecting directly humor containing no insoluble component to a column with letting a mobile phase having a constant composition flow in it, carrying out liquid chromatography with effecting simultaneously molecular sieve and adsorption or distribution. CONSTITUTION:In separating humor components (e.g., plasma, serum, gastric juice, pancreatic juice, bile, etc.) by liquid chromatography, humor containing no insoluble component is directly injected into a column with letting a mobile phase having a constant composition flow in it, and molecular sieve and adsorption or distribution are simultaneously carried out. The operation is preferably carried out under condition wherein a partition coefficient (Kd) shown by the formula (VR is volume of eluation; VO is space volume between gel particles in a packed column; Vi is inner pore volume of gel particles in the packed column) becomes larger with reduction in molecular weight in case of dextran or polyethylene glycol and is in 1.2-5.0 in case of uric acid, the packed column having 2.0-4.0 uniformity HETP/DP (HETP is height equivalent to a theoretical plate; DP is average particle diameter) of gel bed is used, and the humor components are separated.