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Process for the detection of antibodies against spermatozoa by radioimmuno assay or enzymimmuno assay

机译:通过放射免疫测定或酶免疫测定检测抗精子抗体的方法

摘要

In this method allowing to assay for anti-bodies against the sperm, spermatozoids from the donnor or soluble preparations obtained from spermatozoids are first fixed on microtitration PVC plates; said fixed spermatozoids are then incubated with the test serum; then, a radioactively or enzymatically marked binding protein with antibody specificity, e.g. A protein, is bound to those fixed cells, and the fixed radioactivity or enzymatic activity is measured.
机译:在该方法中,可以测定针对精子的抗体,首先将来自供体的精子或从精子中获得的可溶性制剂固定在微量滴定PVC板上;然后将所述固定的精子与测试血清一起温育;然后是具有抗体特异性的放射性或酶标记的结合蛋白,例如蛋白质与那些固定的细胞结合,并测定固定的放射性或酶活性。

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