REAGENT FOR DETERMINATION OF DIRECT-ACTING BILIRUBIN BY THE ENZYMATIC METHOD AND ITS DETERMINATION PROCEDURE

摘要

PURPOSE:A determination of direct-acting bilirubin and a reagent therefor according to the enzymatic method using bilirubin oxidase. CONSTITUTION:The objective reagent for direct-acting bilirubin determination consists of (A) a solution obtained by adding 1-10 units, every time the determination, of bilirubin oxidase, especially originating from Myrothecium microorganism, to a 3.5-4.5pH buffer solution, (B) a 3.5-4.5pH buffer solution free from bilirubin oxidase and (C) a bilirubin standard containing a known concentration of direct-acting bilirubin as a bit. A sample to be determined is added to the buffer solution containing bilirubin oxidase to effect their reaction and the bilirubin is determined by colorimetry.