BIOACTIVE PROTEIN OBTAINED FROM STONE OF ALMOND, APRICOT OR JAPANESE BUSH CHERRY

摘要

PURPOSE:To obtain a protein having strong antiphlogistic and analgesic activities and useful as an antiphlogistic agent, by extracting the stone of almond, apricot (Pruns armeniaca Linn. var. ansu Maxim.) or Japanese bush cherry (Prunus japonica Thunb.) with water. CONSTITUTION:The stones of almond, apricot or Japanese bush cherry are roughly crushed with a mixer, defatted with an oleophilic organic solvent, pulverized, and extracted with water at 10-60 deg.C, preferably at room temperature, for 1-5hr. The extract liquid is dialyzed or subjected to ultrafiltration, and two kinds of proteins are separated and purified from the remaining liquid by chromatography, or the extract liquid is added with a hydrophilic organic solvent, and a protein having high molecular weight is separated from the produced precipitate, and a protein having low molecular weight is obtained from the supernatant liquid. PR-AA having a molecular weight of 300,000-350,000 and PR-BA (10,000-15,000) are obtained from almond, PR-AB (250,000-350,000) and PR-BB (10,000-30,000) from apricot, and PR-AC (250,000-350,000) and PR- BC (10,000-15,000) from Japanese bush cherry.