Method and a base 19 d - luciferin, luciferase and metal ion for the assay of adenosine - triphosphate

摘要

P the invention relates to a method and a reagent for determining the concentration of atp, for example in the conversion reactions of the atp, by contact of the sample to produce, with a reagent luminescence based on - luciferin and luciferase and of magnesium ions, or some other metals, this contact generating a reaction in which the atp and the - luciferin are connected to the luciferase with emission of light, and then by measuring the intensity of the transmitted light, which is a measurement of the concentration of atp. / p & & p & the method consists in using in the determination of one or more competitive inhibitors of the reaction in the form of one or several similar d - luciferin. / p & & p & application to the invention and to the clinical microbiology, as well as for the biological, biochemical and / p