METHOD FOR SEPARATING AND PURIFYING PROTEASES AND ANTIPROTEASES OF BLOOD COAGULATION, AS WELL AS PROTEASE / ANTIPROTEASE COMPLEX

摘要

The present invention relates to a process for separating and purifying the proteases and the anti-proteas. This method is characterized by contacting an insoluble cross-linked polymer having in its chain. THE -SOR- GROUP AND THE -SOR- GROUP IN WHICH R DESIGNATES A HYDROGEN OR METAL ATOM AND R DESIGNATES THE REST OF AN AMINO ACID RELATED TO THE BRIDGE -SO- BY ITS AMINO FUNCTION -NH-, WITH A SOLUTION CONTAINING THE PROTEASES OR THE ANTIPROTEASES OR THEIR COMPLEX, IN THAT THE POLYMER HAVING ADSORPED THE PROTEIN DESIRED IS SEPARATED, IN THAT IT IS CAREFULLY WASHED AT THE BUFFER, IN THAT THE PROTEIN ADSORBED BY A SOLUTION D A POLYCATIONIC COMPOUND IN THE CASE OF T OR BY A SOLUTION OF ALBUMIN IN THE CASE OF AT OR T-AT COMPLEX, AND THAT THE INSOLES THE PROTEIN, IF DESIRED, BY MEANS KNOWN, SUCH AS LYOPHILIZATION IN PARTICULAR. / P P APPLICATION TO THE STUDY OF MECHANISMS OF BLOOD COAGULATION. / P