NOVEL COLIFORM BACILLI HAVING HEAT-RESISTANT BETA-GALACTOSIDASE GENE AND PRODUCTION OF HEAT-RESISTANT BETA-GALACTOSIDASE

摘要

PURPOSE:To produce heat-resistant beta-galactosidase in bacterial cells, by cultivating novel coliform bacilli having a heat-resistant galactosidase gene. CONSTITUTION:A recombinant DNA having DNA carrying genetic information about heat-resistant beta-galactosidase obtained from Bacillus thermophilus IAM 11001 is introduced into a vector plasmid for coliform bacilli to give Escherichia coil 294-43(pHG1) [FERM-P No.7232] and Escherichia coli 294-43(pHG2) [FERM- P No.7233]. The resultant bacteria are then cultivated by the conventional method and collected. The resultant bacterial cells are crushed and extracted to give the aimed heat-resistant beta-galactosidase.