PROBLEM TO BE SOLVED: To obtain the subject new gene having a specific base sequence, coding a heat-resistant β-galactosidase originated from a bacterium belonging to the genus Thermo and useful for the genetic engineering production of the heat- resistant β-galactosidase used as an enzyme for food industry, etc. ;SOLUTION: This new β-galactosidase gene is originated from a bacterium belonging to the genus Thermus, codes heat-resistant β-galactosidase and has a base sequence of the formula or the substantially same function as the base sequence. The new β-galactosidase gene is useful for producing the heat-resistant β-galactosidase catalyzing reactions for hydrolyzing β-(D)1,4-galactoside bonds and used as an enzyme for food industry, etc. The β-galactosidase gene is obtained by separating the heat-resistant galactosidase from a highly thermopile bacterium belonging to the genus Thermus [e.g. Thermus S.P. A4 strain (FERMP-15236)], determining the N-terminal amino acid sequence of the enzyme, and subsequently screening the chromosomal DNA of the A4 strain with a synthesized probe having a base sequence corresponding to the determined N-terminal amino acid sequence.;COPYRIGHT: (C)1998,JPO
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