Procedure to obtain and isolate antibody secreting hybridomas, antibodies and method of obtaining method of obtaining polypeptides

摘要

An antibody characterised by its abilities: to neutralise the HCMVs; to give rise to immunofluororescence-detectable reactions with cells from cultures of human origin infected beforehand with HCMVs and fixed with acetone; to react essentially with a single viral polypeptide with a molecular weight of the order of 94,000, carrying a continuous sequential epitope and capable of being isolated from the cytoplasm of cells of human origin previously infected by HCMVs in the presence of protease inhibitors; preferably to react with protein A. to neut