Procedure of obtaining and Isolate hybridomas secreting Antibodies, Procedure of obtaining method of obtaining Antibodies and polypeptides

摘要

Method of producing hybridomas secreting Antibodies.Comprises: a) reacting to neutralizing antibodies in cultured human cells with a previously infected with HCMV, fixed with acetone and air dried; (b) carry out a First selection of clones giving rise to a reaction of fixationDetectable by immunofluorescence on Human Cells and a Second selection of Monoclonal Antibodies retained at the end of the first Selection that REACT selectively with a viral Protein, induced in the Cytoplasm of infected cells, obtained from this, and co Ntenida fraction in a polypeptide produced from the Cytoplasm.After preliminary separation of Proteins or polypeptides with different Molecular Weights, and (c) recovering the Antibody selected from the reaction products obtained.