Obtaining and the specificity characterization of antibodies against the plant signaling peptide CLE41/44 by gold nanoparticle conjugates

摘要

CLE peptides are important signaling molecules that regulate the balance of proliferation and differentiation of plant cells. In this work, we conjugated gold nanospheres (15 nm) with a chemically synthesized peptide (12 amino acid residues) that the amino acid sequence corresponds to the natural peptides CLE41/44. The "golden number" for CLE41/44 was 2 μg/mL. A stable conjugate was used to produce rabbit polyclonal antibody. By the method of dot analysis, the presence of antibodies to CLE41/44 was detected in antiserum. Using Western blot and ELISA methods, the reaction of the obtained antibody with a conjugate of CLE41/44 with BSA molecules was demonstrated. The obtained antibody is can potentially be used to the quantitative determination and identification of the localization of peptides in plant tissues.