DNA PROMOTER SEQUENCE OF AVIAN TUMOR VIRUS AND USE THEREOF FOR ENHANCED GENE EXPRESSION IN E. COLI.

摘要

ABSTRACT OF THE DISCLOSUREA molecule of double-stranded DNA having a length of about35 base pairs has been removed from the first 150 basepairs at the 3'-end of Avian sarcoma viral DNA. Thismolecule binds to E. coli RNA polymerase and acts as apromoter of gene expression in E. coli. E. coli cellshave been transformed with cloning vehicles which includethis promoter DNA molecule and enhanced gene expressionhas been observed in the transformed cells. By includingthe promoter DNA molecule in cloning vehicles along witha gene associated with the production of a desired chemicalproduct, such as proinsulin, the polypeptide A or B chainsof insulin, the polypeptide portion of interferon, a growthhormone, an enzyme or an antibody, it will be possible toobtain enhanced production of such desired chemical products.