PRODUCTION OF HUMAN IGA SECRETION CELL

摘要

PURPOSE:To produce human IgA secretion cell in high efficiency, by culturing human lymphocyte B in human milk or a medium containing a human milk component. CONSTITUTION:A human lymphocyte B, preferably prepared by removing T cell and macrophage from the lymphocyte separated from peripheral blood, tonsil, spleen, etc., and stimulating and activating the lymphocyte with Staphylococcus aureus Cowan Istrain, is cultured in human milk or a medium containing 1-10% human milk component (fraction precipitated by saturated ammonium sulfate or fraction having a molecular weight of 60,000-90,000 and obtained by the fractionation with a gel-filtration carrier or with an ultra-fitlration membrane) at about 37 deg.C for about 3 days to effect the differentiation and induction of the lymphocyte to the objective IgA secretion cell.