SEPARATED DETERMINATION OF LACTATE DEHYDROGENASE ISOZYME

摘要

PURPOSE:A lactate dehydrogenase inhibitor is added to a solution to be measured on its activity of lactate dehydrogenase activity to enable the measurement of the enzyme activity without separation of its isozymes. CONSTITUTION:When the activity of lactate dehydrogenase (LDH) is determined using nicotinic amide-adenine nucleotide (NAD) as a coenzyme, LDH inhibitor which is separated using an NAD-depending dehydrogenase. The LDH-inhibitor is oftentime contained in NAD standard samples and readily separated from NAD using an NAD-depending enzyme.