Hybridoma cells secreting a monoclonal antibody specific for 5-bromo and 5-iododeoxyuridine and reagents for measuring cellular proliferation

摘要

Cells synthesizing DNA are detected in a rapid, nonradioactive assay using the monoclonal antibody reagent secreted by the hybridoma produced by fusion of a myeloma cell, preferably murine plasmacytoma cell, with an animal antibody producing cell, preferably a mouse spleen cell, immunized with the conjugate of 5-bromouridine or 5-iodouridineand an albumin-carrier molecule. The antibody is specific for 5-bromodeoxyuridine or 5-iododeoxyuridine.