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Method for the enzymatic conversion of a water-insoluble or substantially water-insoluble organic substrate

机译:对不溶于水或基本上不溶于水的有机底物进行酶促转化的方法

摘要

The invention relates to a method of converting a water-insoluble or substantially water-insoluble organic substrate under the influence of a water-soluble enzyme requiring a cofactor. The conversion is carried out in a micellar system, comprising an organic solvent which is not or substantially not water-miscible and in which the enzyme is solubilized via reversed micelles, stabilized by one or more surfactants, while the cofactor is regenerated in situ by means of one or more second enzymes also solubilized via reversed micelles in the organic solvent.
机译:本发明涉及一种在需要辅因子的水溶性酶的影响下转化不溶于水或基本上不溶于水的有机底物的方法。转化在胶束体系中进行,该胶束体系包含与水不混溶或基本上不与水混溶的有机溶剂,其中酶通过反胶束增溶,并用一种​​或多种表面活性剂稳定,而辅因子通过以下方式原位再生:一种或多种第二种酶也可通过反胶束溶于有机溶剂。

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