A method for producing a complement component Clq to which a substance is bound and a measurement method using the complement component Clq

摘要

A method for preparing a substance-conjugated complement component Clq is presented. Thus, 1mg -D-galactosidase obtained from E. coli was dissolved in 0.2ml 0.1M-phosphate buffer (pH=6.0), reacted with 0.1mg N, N'-O-phenylenedimaleimide in 0.2ml 0.1M-phosphate buffer (pH=6.0) at 30C for 25min, and passed through a sephadex G-35 column to give 720 g maleimide -Dgalactosidase, which was reacted with complement component clq at 4C for 48hr and passed through sepharose 6B column to give 1.6mg -D-galactosidase-conjugated complement component Clq.