TWO-DIMENSIONAL SEPARATION HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

PURPOSE:To make it possible to simultaneously perform the separation of a specimen component by the filler of a column and that by a DC electric field, by applying a DC electric field to a column into which a mobile phase containing a liquid specimen is introduced. CONSTITUTION:A liquid specimen is separated on the basis of a holding time by the filler of a column 5 while, by the DC electric field applied to the column 5, the component of the specimen having net negative charge is attracted to the positive pole side of the DC electric field and the component of the specimen having net positive charge is attracted to the negative pole side of the electric field. By this method, the component of the specimen separated on the basis of the same holding time by the filler and the components of the specimen having net positive and negative charges separated by the DC electric field reach the outlet side of the column 5. Said components are further introduced into minute cells C1-Cn through fine tubes 71-7n and white beams B1-Bn are projected to said components through slits S1-Sn. The absorbancies thereof are measured by photodiode arrays P1-Pn to be inputted to a data processor 10. By this method, analytical results can be made accurate.