COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD FOR THE DETECTION OF ANTIBODIES

摘要

Abstract of the DisclosureA competitive enzyme-linked immunosorbent assay(ELISA) for the detection of antibodies is disclosed.This ELISA technique is more sensitive, more specific,and more accurate than known ELISA techniques. The com-petitive ELISA of this invention is particularly suitedto the detection of human T-cell leukemia-lymphoma virustype III (HTLV-III). An improvement of the ELISAtechnique represented by the present invention lies inthe use of a reaction buffer which suppresses non-specific adsorptive reactions between the test substrateand the test antigen. Furthermore, the ELISA techniqueis improved by removing contaminants from the targetantigen preparation.