Method of preparing Vaccines capable of Inducing immunity to Moraxella bovis, which has applications for the vaccination of cattle.Includes culturing cells of Moraxella bovis in a medium containing a substrate induces the production of protease and other Nutrients such as RPMI 1640 with Amine, N - Z 2% and 0.2% sodium bicarbonate at a temperature between 30jc and 35jc, until the absorbanci To 590 nm is less than 2.0 Absorbance Units; deactivation of cultivation by Treatment with an agent to form a desactivante Bacterin; and combination of Bacterin with an adjuvant to prepare.
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