Effect of Chicken Akirin2 Gene on Immune Response Induced by VP2 DNA Vaccine of Infectious Bursal Dis- ease Virus

摘要

In order to investigate the effect of chicken Akirin2 gene on the immune response induced by VP2 DNA vaccine of infectious bursal disease virus( IBDV). [Methods] The 14-day-old SPF chickens were immunized with recombinant plasmids expressing VP2 protein and Akirin2 protein,and strengthened immunization was conducted at the 14 thday after the first immunization. Finally,test chickens were challenged with IBDVBC6-85 virulent strain. [Resultss]Test results showed that Akirin2 gene could enhance the specific immune response induced by VP2 DNA vaccine,improve the proliferation of peripheral blood lymphocytes and affect the expressing of cytokines TNF-α,IFN-γ,IL-1β,IL-2,IL-4,IL 6,IL-9,IL-10,IL-17 and IL-18. Effects of recombinant plasmids co-expressing Akirin2 protein and VP2 protein on cytokine expression showed some differences with the recombinant plasmids expressing Akirin2 protein or VP2 protein along. [Conclusions] Chicken Akirin2 gene could significantly enhance the humoral immune response and cellular immune response induced by VP2 DNA vaccine of IBDV.