HUMAN MANGANESE SUPEROXIDE DISMUTASE PRODUCED BY GENETIC ENGINEERING

摘要

A double-stranded cDNA molecule which includes DNA encoding human manganese super oxide dismutase has been created. The sequence of one strand of a double- stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such mole- cules may be introduced in procaryotic, e.g., bacteri- al, or eukaryotic, e.g. , yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions sa as to produce human manganese superoxide dismutase or analogs thereof which may then be recov- ered. Human MnSOD or analogs thereof may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of iso- lated organs, or treat inflammations. The invention also concerns a method of producing enzy- matically active human manganese superoxide dismutase or an analog thereof in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase by maintaining the bacterial cell under suitable conditions and in a suitable pro- duction medium. The production medium is supplemented with an amount of Mn++ so that the concentration of Mn++ in the medium is greater than about 2 ppm. This invention also concerns a method of recovering purified enzymatically active manganese super oxide dismutase from bacterial cells.