Competitive immunoassay protocal for targets including lipoproteins and alpha-fetoprotein

摘要

The invention relates to a process for the immunoassay of a substance, such as an antigen, a hapten or an antibody.PP This process comprises the following stages:PP(1) contacting a sample containing the substance to be assayed with a labelled immunoactive reagent specific to said substance, the immunoactive reagent quantity being such that the substance to be assayed is in excess compared with that labelled immunoactive reagent;P P(2) contacting at least part of the thus obtained reaction medium with a solid phase, to which is fixed an immunoactive reagent specific to the substance to be assayed;PP(3) separating the reaction medium from the solid phase; andPP(4) determining the labelled immunoactive reagent content of the solid phase. P PThis process is more particularly applicable to the assaying of lipoprotein and in this case use is made of an antibody labelled by an enzyme, such as the Raifort peroxidase. It is also possible to assay . alpha.-foetoprotein by using monoclonal antibodies.