FLUORO-POLARIZING IMMUNOASSAY METHOD OF AMPHETAMINE AND METHAMPHETAMINE

摘要

This disclosure relates to a method and reagents for determining amphetamine and methamphetamine in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross-reactants such as &bgr;-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4. 0 to about 7.5 without adjustment to an alkaline pH.