PROCESS TO PRODUCE A SELECTED POLYPEPTIDE IN A PROPER HOST INSECT CELL; RECOMBINATOR VECTOR, INFECTED INSECT CELL, PEPTIDE; POLYPEPTIDE MOLECULA; MODIFIED HOST, SURFACE ANTIGEN OF HEPATITIS B VIRUS

摘要

A method for producing in a suitable insect host cell a selected polypeptide which comprises isolating a first DNA segment including a viral promoter from a virus capable of infecting said insect host cell, isolating from a suitable source a second DNA segment containing the sequence encoding said polypeptide, combining said first and second DNA segments to form a continuous strand of a third DNA segment including vector DNA in which said second DNA segment lies adjacent to and is in-frame with said promoter of said first DNA segment and contains transcription termination signals, forming a recombination vector by effecting recombination between said third segment and baculovirus genomic DNA, contacting said recombination vector so produced with said insect host cell under conditions such that said recombination vector segment becomes incorporated into said insect host cell to produce a treated insect host cell, and growing the host cell so treated and isolating the selected polypeptide from said host cell or culture fluids. Further, a method for producing in a suitable insect host cell Hepatitis B virus surface antigen.