New potato root eel-worm proteins - and antibodies useful for determn. of eel-worm populations in soil

摘要

Type-specific thermostable proteins isolated from eggs, larvae or adult potato root eelworms (Globodera rostochiensis and G. pallida) are new. Also claimed are polyclonal and monoclonal antibodies to such proteins. G. rostochiensis proteins have molecular wts. of 20.8 (+-) 1.0, 20.6 (+-) 1.0, 18.0 (+-) 1.0 and 18.0 (+-) 1.0 kD and isoelectric points of 5.2 (+-) 0.1, 5.3 (+-) 0.1, 6.0 (+-) 0.1 and 5.7 (+-0.1 respectively. G. pallida proteins have molecular wts. of 21.0 (+-) 1.0, 20.5 (+-) 1.0, 17.0 (+-) 1.0 and 17.0 (+-) 1.0 kD and isoelectric points of 5.32 (+-) 0.1, 5.4 (+-) 0.1, 5.8 (+-) 0.1 and 5.6 (+-) 0.1 respectively. The antibodies may be labelled, e.g. with enzymes. Determn. of eelworm populations is effected by isolating an eelworm protein homogenate from a soil sample, opt. heat-treating the homogenate and removing denatured proteins, and analysing the prod. using the antibodies, e.g. by the sandwich ELISA method.