FREMGANGSMAATE FOR FREMSTILLING OG SELEKSJON AV EN REKOMBINANT BAKTERIOFAG SOM INNEHOLDER ET GENETISK FRAGMENT OG KODER FOR ALFA-AMYLASE, EGNET FOR BRUK I HETEROLOG TRANSFORMERING AV EN BACILLUS-VERTS-MIKROORGANISME.

摘要

A recombinant bacteriophage, a method for producing and selecting the recombinant bacteriophage and a method for heterologous cloning of DNA are disclosed. The recombinant bacteriophage is produced by ligating genetic fragments encoding a desired genetic trait with DNA from a bacteriophage, incubating with DNA from a second Bacillus microorganism prototrophic for a growth requirement, incubating with a host Bacillus auxotrophic for the growth requirement. Transformed host Bacillus are selected by growing the mixture on a growth medium which does not contain the growth requirement and determining the presence of the genetic trait. The recombinant bacteriophage containing the desired genetic trait is recovered from the host Bacillus by induction. Heterologous cloning can be accomplished by incubating a host Bacillus with the recombinant bacteriophage.