FLUOROMETRIC OR ELECTROCHEMICAL ASSAY OF COMPOUNDS HAVING PRIMARY AMINO GROUPS; ADDUCTS OF NAPHTHALENE-2,3-DICARBOXALDEHYDE, PRIMARY AMINE AND CYANIDE ION

摘要

A method for the fluorometric or electrochemical assaying of low levels of primary amines and more particularly, biogenic and endogenous amines such as catechol amines, amino acids, and peptides, by reacting a substituted or unsubstituted naphthalene-2-3-dicarboxyaldehyde with the non-fluorescing primary amine of interest in the presence of a cyanide ion under mildly alkaline conditions thereby to form an entity which is amenable to both fluorescent and electrochemical detection.