Monoclonal antibodies with cross-reactivity and protection against P. aeruginosa serotypes.

摘要

A process for the preparation of a human monoclonal antibody capable of recognizing multiple serotypes of psedomonas aeruginosa. These antibodies are capable of binding to the molecules of lipopolysaccharide selected strains of pseudomonas aeruginosa and are protective against lethal attacks of this microorganism. Antibodies of the invention may be obtained by transformation of b lymphocytes from donor exposed to pseudomonas aeruginosa with epstein-barr virus, or fusion of a cell line inmortal with human b lymphocytes for a hybridoma expressing said antibodies or for isolation of genes encoding such antibodies and then insertion into an expression vector in a host cell and proper. Antibodies are suitable for treatment infection caused by great-negative microorganisms and in addition may be used in equipment and a method for detecting the presence of blood sample peudomonas ina aeruginosa. (Machine-translation by Google Translate, not legally binding)