Process for the production of Monoclonal Antibodies against factor inhibition of Migration of human macrophages.

摘要

Method to obtain Monoclonal Antibodies against factor inhibition of Migration of human macrophages.Consists in cultivating these hybridoma cells producing Monoclonal Antibodies to Physiological temperature in Culture Medium under Air enriched with CO2, and isolating Antibodies from Culture supernatant by Ammonium sulphate precipitation and chromatographic purification and, IF You want to,Transform a monoclonal antibody obtained a derivative thereof with Biotin - - hidroxisuccinimidilester Biotin Derivatives. They are useful for Qualitative and quantitative determination of Human MIF in biologicosy Fluids on surfaces of cells for the purification of Human MIF, and their individual proteins as Therapeutic means.