SA-11 ROTAVIRUS MAJOR OUTER CAPSID PROTEIN (VP7) IN BACULOVIRUS-INSECT CELL EXPRESSION SYSTEM

摘要

The gene coding for SA-11 rotavirus major outer capsid protein (VP7) was cloned and expressed in a baculovirus-insect cell expression system. The protein, which is antigenically similar to native VP7 protein, has a molecular weight of 34 kilodaltons and is secreted into the tissue culture media. The recombinant VP7 protein is reactive with neutralizing antiserum prepared against SA-11 virus and can be utilized in a vaccine, as an antigenic preparation for producing antibodies or in a diagnostic assay.