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Process for preparing uridine diphosphate-N-acetylgalactosamine

机译:尿苷二磷酸-N-乙酰半乳糖胺的制备方法

摘要

A process for preparing uridine diphosphate-N-acetylgalactosamine, which comprises treating a reaction solution obtained by the enzymatic conversion of uridine diphosphate-N-acetylglucosamine to uridine diphosphate-N-acetylgalactosamine, with uridine diphosphate-N-acetyt- glucosamine pyrophosphorylase to decompose the remaining uridine diphosphate-N-acetylglucosamine in the solution and then separating therefrom the uridine diphosphate-N-acetylgalactosamine for purification. In one aspect of this invention, it relates to a method for measuring the activity of a-N-acetylgalactosaminyl transferase characterized by the use of said reaction solution as the substrate for the transferase.
机译:一种制备尿苷二磷酸-N-乙酰半乳糖胺的方法,该方法包括用尿苷二磷酸-N-乙酰基-葡糖胺焦磷酸化酶处理通过将尿苷二磷酸-N-乙酰氨基葡糖酶转化为尿苷二磷酸-N-乙酰半乳糖胺而获得的反应溶液。溶液中剩余的尿苷二磷酸-N-乙酰氨基葡糖,然后从中分离出尿苷二磷酸-N-乙酰半乳糖胺以进行纯化。在本发明的一个方面,它涉及一种测量α-N-乙酰半乳糖胺基转移酶活性的方法,其特征在于使用所述反应溶液作为转移酶的底物。

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