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Method for controlling foreign gene expression by controlling sugar concentration of medium and method for producing foreign gene product
Method for controlling foreign gene expression by controlling sugar concentration of medium and method for producing foreign gene product
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机译:通过控制培养基的糖浓度来控制外源基因表达的方法和生产外源基因产物的方法
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摘要
Escherichia coli carrying a hybrid plasmid constructed by inserting a desired foreign gene into an expression vector, was cultured in a medium containing a sugar component utilizable by the E. coli as a carbon source at a concentration of 0.3% or more so that the expression of said desired foreign gene was suppressed. This E. coli was cultured in the medium in which the sugar concentration was maintained at 0.3% or more in a first process to suppress the foreign gene and to support sufficient cell growth and thereafter at less than 0.3% in a second process to release the suppression to permit effective production of the foreign gene product, which resulted in high concentration of the foreign gene product in the final culture.
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