METHOD FOR TRANSPLANTING A CORE OF A DONOR CATTLE EMBRYO IN A RECOVERY OVOCYTE, AND BOVINE EMBRYO PRODUCED THEREBY

摘要

The invention relates to a method for transporting cattle donut embryo nuclei into encrusted egg extractants. According to the invention, the process comprises the following steps: A) isolating an encircled kernel A MEMBRANE FROM A CELL OF THE DONOR EMBRYO; B) REMOVING NUCLEAR CHROMOSOMAL MATERIAL FROM THE OVOCYTE RECEIVER (TO PREPARE AN ENUCLEATED RECOVERY OOCYTE); C) PLACE THE DONOR CORE SURROUNDED BY A MEMBRANE HAVING ITS MEMBRANE ADJACENT TO THAT OF THE OVOCYTE ENUCLEE RECEIVER; AND D) ELECTRICALLY FUSING THE MEMBRANES OF THE CORE SURROUNDED BY A MEMBRANE AND THE RECOVERED ENUCLEOUS OVOCYTE TO FORM A SINGLE EMBRYONIC CELL WITH A CORE FROM THE DONOR EMBRYO. / P P THE INVENTION APPLIES IN PARTICULAR FOR THE MULTIPLICATION AND CLONING OF BOVINE EMBRYOS BY NUCLEAR TRANSFER. / P