AMINO ACID SEQUENCE OF L-PHENYLALANINE AMMONIA LYASE, ITS STRUCTURAL GENE, NOVEL BASE SEQUENCE CONTAINING SAME, TRANSFORMANT PRODUCED THEREWITH AND PRODUCTION OF L-PHENYLALANINE USING SAME

摘要

PURPOSE:To provide an amino acid sequence of L-phenylalanine ammonia lyase (PAL) enabling the production of PAL useful for the production of L- phenylalanine without using expensive amino acids, and its structural gene. CONSTITUTION:A yeast Rhodosporidium toruloides is cultured under a PAL- inducing condition and an mRNA fraction of PAL is separated from the cultured cell. A single stranded cDNA is prepared from the mRNA with a transcriptase and converted to a double stranded cDNA. The DNA is inserted into a vector, the vector is transformed into a host cell and a cDNA library is prepared therefrom. A cDNA coding a PAL structural gene is cloned from the library to obtain a DNA coding PAL structural gene. Said DNA is inserted between a 3'-terminal of a promoter region and a 5'-terminal of a terminator region and a host cell is transformed by using the resultant recombinant DNA. The transformant is cultured to produce PAL.