PRODUCTION OF EYE DROP TREE BY TISSUE CULTURE

摘要

PURPOSE:To enable the production of a large amount of the eye drop tree (Acer nikoense Maxim) in a shortened time, by inducing callus by culturing living tissues of the eye drop tree on the solid medium, and growing the callus by culturing. CONSTITUTION:Cut pieces of a living tissue of eye drop tree such as pieces of stem, leaf, preferably petioles, are cultured in a solid medium such as the MURASHIGE-SCOUGUE to induce callus. In order to accelerate the induction into callus, auxin and cytokinin may be added to the culture medium in an amount of 10-7-10-5 M. Then, the callus is cultured in a liquid medium by shaking or stirring process, and the callus becomes blocks, disperses in the medium and propagates. Then, the calluses are separated and dried. Thus, the mass production of the eye drop tree becomes possible regardless of the natural conditions such as seasons, weather and soils.