UTILIZATION OF INORGANIC SUBSTRATA AND PRODUCTION OF ORGANIC SUBSTANCES BY NON-DIFFERENTIATED PLANT TISSUE CULTURES CULTIVATED IN LOW MAGNETIC FIELD

摘要

In order to survey how magnetic fields affect the mineral nutrition and the primary metabolism of plant tissue cultures, calluses of soybean (Glycine max), sunflower (Helianthus annuus) and potato (Solanum tuberosum) were incubated under low magnetic field (LMF) and also in normal geo-magnetic environment for 48 hours in a short time exposure, and for 500 hours, in a long run respectively. The substrate utilization related to the main organic synthesis in the non-differentiated tissue cultures were determined in order to established the turnover rate in a self-supporting system. At short time exposure the growth of potato calluses was stimulated but not the other two types of calluses. The nitrogen utilisation, in both oxidised and reduced form, is higher for all the tissues maintained in the shield box. The phosphorus utilisation is higher for the sunflower tissues and lower for potato and soybean tissues. At long time exposure the nitrogen utilisation remain higher in shielding conditions but the phosphorus utilisation decrease under the control. The iron accumulation in the shielded tissues is higher than in control one. The lipids and carbohydrates content decreases compared to the control.