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A process for preparing an alpha-galactosidase enzymatic preparation and procedure for the hydrolysis of raffinose during the use of this enzymatic preparation

机译:制备α-半乳糖苷酶的酶制剂的方法以及在使用该酶制剂期间水解棉子糖的方法

摘要

The invention relates to a process for the production of the alpha-galactoxidase enzyme by culturing yeasts of the Saccharomyces cerevisiae genus in a temperature range from 20 DEG C. to 40 DEG C. The invention also relates to a process for enzymic hydrolysis of raffinose by alpha-galactoxidase from Saccharomyces cerevisiae. Such hydrolysis may take place with the yeast cells being present or also in the presence of enzymic extracts, both as such and enriched. An important advantage of the invention is the high alpha-galactoxidasic activity of the selected microorganisms, together with the absence of any invertasic activity.
机译:本发明涉及一种通过在20℃至40℃的温度范围内培养酿酒酵母属的酵母来生产α-半乳糖氧化酶的方法。本发明还涉及一种通过如下方法对棉子糖进行酶促水解的方法:来自酿酒酵母的α-半乳糖氧化酶。这种水解可以在存在酵母细胞的情况下或在酶提取物本身存在或富集的情况下进行。本发明的一个重要优点是所选择的微生物具有高的α-半乳糖氧化活性,并且不存在任何转化活性。

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