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Method for the determination of an allergy and method for the specific determination of the allergen responsible for the allergy
Method for the determination of an allergy and method for the specific determination of the allergen responsible for the allergy
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机译:确定过敏的方法和具体确定引起过敏的过敏原的方法
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摘要
The present invention provides a process for the detection of the presence of an allergy and for the specific detection of the allergen responsible for the allergy, based on the cellular principle, wherein leukocytes of a sample to be investigated are incubated with an allergen or another stimulation factor of degranulation, such as anti-IgE, in an aqueous medium, the leukocytes are then separated off and the remaining solution is incubated with a compound of the general formula: Y-X-C in which Y is an amino acid or a peptide containing 2 or 3 amino acids, the amino acids optionally carrying a conventional protective group on the amino end, X is arginine or lysine and C is a chromogenic residue, in the presence of a buffer effective at pH 6.3 to 8.0 and the liberation of the chromogenic residue is measured.
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