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Separation of large DNA molecules in alternating asymmetric electric fields
Separation of large DNA molecules in alternating asymmetric electric fields
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机译:在交替不对称电场中分离大DNA分子
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摘要
DNA molecules are separated by a gel electrophoresis method which employes alternating higher and lower strength, electric fields applied in opposite directions to a gel matrix containing DNA. The higher strength field is maintained for a shorter interval than the lower strength field, and the ratio of the product of the lower strength field and its pulse interval to the product of the higher strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the lower strength field.
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