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Separation of large DNA molecules in alternating asymmetric electric fields
Separation of large DNA molecules in alternating asymmetric electric fields
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机译:在交替不对称电场中分离大DNA分子
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摘要
A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix containing DNA. The high strength field is maintained for a shorter interval than the low strength field, and the ratio of the product of the low strength field and its pulse interval to the product of the high strength field and its pulse interval is greater than 4:1 and sufficient to produce a net migration of the DNA molecules in the direction of the low strength field.
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