METHOD FOR DETERMINING THE BLOOD GROUP WITH THE SOLID-PHASE METHOD USING ANTI-BODY-SPECIFIC ANTIBODIES OF THE IGM TYPE, AND CARRIER AND KIT FOR CARRYING OUT THE METHOD

摘要

A method for determining blood groups by the solid-phase method using blood group-specific antibodies and a carrier to whose surface the antibodies can be irreversibly bound is obtained by a solution of an antibody of the IgM type… (a) either being contacted with the carrier surface simultaneously with the erythrocyte suspension to be determined or… (b) the antibodies first being dried as reagent reservoir on the carrier surface and being dissolved, and thus contacted with the carrier surface, only by the addition of the erythrocyte suspension when the assay is carried out,… and the determination being carried out a short time after the contacting, it being necessary that the antibody concentration is no higher than required to form an irreversible or immobilised layer of antibody molecules on the carrier surface, which layer is able specifically to bind erythrocytes, and is insufficient to bring about an agglutination reaction.