PROCESS FOR DETERMINING A PROTEIN ACCORDING TO THE PRINCIPLE OF THE FLUORESCENCE POLARIZATION IMMUNOASSAY

摘要

To determine a protein in a homogeneous system, a sample solution is incubated simultaneously with… a) a peptide sequence which is labelled with a fluorescent compound and is composed of 6 to 14 amino acids, of which two are cysteine, which together form a disulphide bridge, the sequence corresponding to an epitope sequence of the protein to be determined and… b) an antibody which is able specifically to bind both to the protein and to the peptide sequence and has molar relative affinities for the fluorescent peptide sequence of the protein and for the native protein which differ by not more than a factor of 6, and the depolarisation of the polarised light passed through the incubated solution is measured. …IMAGE…