Process for the determination of a protein according to fluorescence polarization immunoassay principle

摘要

The present invention provides a process for the determination of a protein according to the fluorescence polarization immunoassay principle in a homogeneous system by simultaneous incubation of the sample solution withPPa) a peptide sequence, labelled with a fluorescing compound, of 6 to 14 amino acids, two of which are cysteine which form a disulphide bridge with one another, the sequence thereby corresponding to an epitope sequence of the protein to be determined, andPP b) an antibody which is specifically bindable not only with the protein but also with the peptide sequence and displays for the fluorescing peptide sequence of the protein and the native protein molar relative affinities which differ by a factor of at most 6, and measurement of the depolarization of polarized light passed through the incubated solution.