PROTEASE-PRODUCING BACTERIUM, PROTEASE PRODUCED FROM THE SAME BACTERIUM AND PURIFICATION THEREOF

摘要

PURPOSE:To provide the subject protease-producing bacterium having heat resistance and an optimum pH of the alkaline side and capable of producing a protease which can be readily separated and purified in a high yield. CONSTITUTION:A protease-producing bacterium belonging to Nocardiopsis genus, having cell walls of meso-form by the 2,6-diaminopimelic acid analysis and of III/C type and containing PIII type phospholipid. The above-mentioned bacterium is separated from the soil in Kyoto Prefecture, named Nocardiopsis sp. OPC-210 strain and committed as FERM P10508 to Bikoken. By preparing an acetone powder from the resultant OPC-210 strain, dissolving the prepared acetone powder in a weakly acidic solution, dialyzing the solution and purifying the dialyzed solution using the column chromatography, the objective protease of high purity can be obtained in a high yield.