SEPARATION OF SUBSTANCE FOR ANALYSIS BOUND WITH COMBINED PROTEIN AND PREPARATIVE REAGENT FOR SAMPLE FOR MEASURING VITAMIN B12 IN SERUM

摘要

The method entails the binding protein being degraded by a thiol which cleaves SH groups in the alkaline range and is characterised in that a 1,2-dithiolane-3-carboxylic acid of the formula (I) IMAGE in which n is an integer from 1 to 8, is used as thiol which cleaves SH groups, and the method is used to prepare samples for analytical and investigative methods for determining the liberated analyte, in particular for immunoassays for vitamin B12.