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Method for separating analyte bound to binding protein and sample preparation reagent for measuring vitamin B12 in serum
Method for separating analyte bound to binding protein and sample preparation reagent for measuring vitamin B12 in serum
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机译:结合蛋白结合物分析物的分离方法及测定血清中维生素b12的样品制备试剂
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摘要
The method entails the binding protein being degraded by a thiol which cleaves SH groups in the alkaline range and is characterised in that a 1,2-dithiolane-3-carboxylic acid of the formula (I) IMAGE in which n is an integer from 1 to 8, is used as thiol which cleaves SH groups, and the method is used to prepare samples for analytical and investigative methods for determining the liberated analyte, in particular for immunoassays for vitamin B12.
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