SYNTHETIC INTRON DNA, RECOMINANT DNA CONTAINING THE SAME AND TRANSFORMANT TRANSFORMED THEREWITH

摘要

NEW MATERIAL:A synthetic intron DNA, having a region containing the 5' and 3'-terminal consesus sequences and a region containing a sequence of a branching part, either of at least one set of recurring base sequences in the opposite directions being a sequence of region containing a consesus sequence and/or a region containing the branching part and at least one of the above- mentioned one set of the base sequences in the opposite directions, present in base sequences other than the above-mentioned regions and sandwiching regions containing the sequence of the branching part. USE:A genetic expression controlling agent and reagent for elucidating a splicing mechanism. PREPARATION:For example, a synthetic intron is linked to a plasmid pUC118 and cloned to provided a plasmid pUC-INTRON, which is then cleaved with restriction enzymes SnaBI and PstI to afford DNA fragments. The resultant DNA fragments are then linked to a DNA fragment cleaved with restriction enzymes XbaI and PstI using a T4DNA ligase to provided a synthetic intron DNA.