HYBRID CHLORELLA CELL PRODUCED FROM LIMNETIC AND MARINE CHLORELLA CELL AND METHOD FOR CELL FUSION THEREOF

摘要

PURPOSE:To obtain the subject cell having both characteristics of a high propagation rate and high eicosapentaenoic acid(EPA) content by converting limnetic chlorella cells and marine chlorella cells into protoplasts, fusing the resultant protoplastic cells and then regenerating cell walls. CONSTITUTION:Limnetic chlorella cells 1 and marine chlorella cells 4 are initially converted into protoplasts and hard cell walls 3 covering the outside of both the cells are dissolved to provide only protoplasts 2. The obtained limnetic chlorella protoplastic cells 6 and marine chlorella protoplastic cells 7 obtained by the conversion into the protoplasts are subjected to cell fusion and the cell walls 3 are then regenerated on the outside of the fused cells to create hybrid chlorella cells 11.