SELECTIVE SEPARATION AND PURIFICATION OF DOCOSAHEXAENOIC ACID AND EICOSAPENTAENOIC ACID FROM INTERNAL ORGANS OF CUTTLEFISH

摘要

PURPOSE:To selectively obtain docosahexaenoic acid and eicosapentaenoic acid in high efficiency by bringing a lyophilized product of the internal organs of cuttlefish into contact with CO2 being in a state higher than both its critical temperature and pressure followed by passing through a column packed with silver nitrate-carrrying powder. CONSTITUTION:A lyophilized product of the internal organs of cuttlefish is brought into contact with CO2 inert thereto under conditions higher than both its critical temperature (pref. by 1-50 deg.C) and critical pressure (pref. by 1-150kg/cm2) to migrate the lipids contained in said organs into the CO2. Thence, the resultant CO2 is passed through a column packed with porous inorganic powder such as of silica gel or alumina with silver nitrate carried thereon to separate the objective docosahexaenoic acid and eicosapentaenoic acid from the above lipids.